Those marked “(H)” are recorded in history.
Knights Hospitaller of St John[]
- (H) Juan de Homedès, Grand Master of the Order
- (H) Nicholas Durand de Villegagnon, Knight of the Order
- Graham Reid Malett, (Gabriel) Grand Cross of Grace and Bailiff of the Order
- (H) Leone Strozzi, Prior of Capua and Commander of the King of France’s Mediterranean fleet
- (H) Francis of Lorraine, Grand Prior in France of the Order, and brother of the Scottish Queen Dowager
- Jerott Blyth, of Scotland and Nantes, Knight of the Order
- (H) Galatian de Césel, Knight of the Order and Governor of Gozo
- (H) Nicholas Upton, Turcopilier or Officer of the English Tongue within the Order
- (H) des Roches, Serving Brother at the Châtelet, Tripoli
- (H) Michel de Seurre, Sieur de Lumigny, Knight of the Order
- (H) George Adorne, Bailiff of the Order and Governor of Mdina
- (H) Marshal Gaspard de Vallier, Knight of the Order and Governor of Tripoli
- (H) James Sandilands of Calder, Preceptor-General of the House of Torphichen of the Order in Scotland
France[]
- (H) Anne de Montmorency, Marshal, Grand Master and Constable of France
- (H) Piero Strozzi, Seigneur de Belleville, Count de Languillara, Florentine colonel of infantry under the King of France and brother of Leone Strozzi
- (H) Gabriel de Luetz, Baron et Seigneur d’Aramon et de Valabrègues, French Ambassador to Turkey
- (H) Henri Cleutin, Seigneur d’Oisel et de Villeparisis, French Ambassador and Lieutenant-General to the King of France in Scotland
- (H) Nicolas de Nicolay, Sieur d’Arfeville et de Bel Air, cosmographer to the King of France
Ireland[]
- (H) Cormac O’Connor, heir to Brian Faly O’Connor, rebel Irish chieftain against England
- Oonagh O’Dwyer, his former mistress
- (H) George Paris, an agent between Ireland and France
Scotland[]
- Francis Crawford of Lymond, Comte de Sevigny
- Richard Crawford, Baron Culter, his brother
- Sybilla the Dowager Lady Culter, his mother
- Mariotta, Richard’s Irish-born wife
- Kevin Crawford, Master of Culter, Richard’s infant son
- (H) Walter Scott of Buccleuch, Warden of the Middle Marches and Justiciar of Liddesdale
- (H) William Scott of Kincurd, Younger of Buccleuch, his heir
- (H) Janet Beaton, Lady of Buccleuch, his wife
- (H) Grizel Beaton, Lady (Younger) of Buccleuch, sister to Janet Beaton and wife to Will Scott
- (H) Robert Beaton of Creich, Captain of Falkland, their brother
- (H) Mary of Guise, Queen Mother of Scotland, and mother of the child Mary, Queen of Scots
- (H) Walter Kerr of Cessford and (H) John Kerr of Ferniehurst, heads, respectively, of the two main branches of the important Scottish lowland family of Kerr, at feud with the Scotts
- (H) Peter Cranston of Cranston, a Border landowner, neighbour of the Kerrs and the Scotts
- Joleta Reid Malett, sister to Sir Graham Reid Malett
- Evangelista Donati of Venice, Joleta’s governess and duenna
- (H) Thomas Erskine, Master of Erskine, Chief Scots Privy Councillor and Special Ambassador
- (H) Margaret Fleming, his wife
- (H) Jenny Stewart, Lady Fleming, mother to Margaret Fleming, and former mistress to the King of France
- Men and officers of St Mary’s:
- Adam Blacklock, artist
- Fergie Hoddim of The Laigh, lawyer
- Randolph Bell, (Randy) physician
- Alexander Guthrie, lecturer and humanist
- Hercules Tait, diplomat and antiquarian
- Lancelot Plummer, engineer and architect
- Archie Abernethy, former Keeper of the French King’s menageries
- Thomas Wishart, (Tosh) acrobat
- (H) John Thompson, (Jockie, Tamsín) sea rover
- (H) Hough Isa, a friendly lady open to barter
England[]
- Kate Somerville, mistress of Flaw Valleys
- Philippa Somerville, her daughter
- Cheese-Wame Henderson, their servant
Turkey[]
- (H) Dragut Rais, Anatolian corsair in Turkish service
- (H) Salah Rais, corsair in Turkish service and joint lieutenant with Dragut
- (H) Sinan Pasha of Smyrna, General in command of Sultan Suleiman’s expedition
- (H) The Aga Morat, Lord of Tagiura and ally of the Sultan Suleiman
- Salablanca a Moor from Spain enslaved by the Knights
- Güzel, companion to Dragut Rais
- Kedi, a nurse
Heralds[]
- (H) William Flower, Chester Herald (England)
- (H) Adam Maccullo, Bute Herald (Scotland)
- (H) Robbie Forman, Ross Herald (Scotland)